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KMID : 1094020110280010075
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Journal of Veterinary Clinics
2011 Volume.28 No. 1 p.75 ~ p.80
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Proteome Analysis of Pigs Fed with Tissue Culture Medium Waste after Harvest of Korean Wild Ginseng
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Seol Jae-Won
Chae Joon-Seok
Kang Hyung-Sub
Kang Chun-Seong
Ihn Dong-Chul
Park Sang-Youel
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Abstract
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Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in pigs fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n = 30/group) of pigs were administered with 0 (control) and 16 ml/L (treatment) TCM-KWG through drinking water. After 4 weeks, we examined the protein expression pattern of longissimus dorsi muscle by Two-dimensional electrophoresis analysis. TCM-KWG treatment significantly increased two spot’s density, and markedly reduced one spot’s density in the muscles. We identified 3 proteins (heat shock protein 90-alpha, myosin binding protein and cofilin 2) by the ESI-MS/MS (Q-TOF2, Micromass). These results demonstrate that TCM-KWG treatment may play a protection role against physiological stress in pigs, like as increased heat shock protein 90-alpha.
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KEYWORD
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Gingseng, TCM-KWG, Proteomics, Heat shock protein, Cofilin
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